1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Alpha 1 Antichymotrypsin
  6. Serpin A3 蛋白, Human (HEK293, His)

Serpin A3 蛋白, Human (HEK293, His)

目录号: HY-P73412
COA 产品使用指南

Serpin A3 蛋白是 14 号染色体上的丝氨酸蛋白酶抑制剂,通过组织特异性多态性影响蛋白酶靶向。序列变异与阿尔茨海默病有关,而缺陷则与肝脏疾病有关。Serpin A3 蛋白, Human (HEK293, His) 是重组的 丝氨酸蛋白酶抑制剂 A3 蛋白,由 HEK293 表达,带有 C-His 标签。Serpin A3 蛋白, Human (HEK293, His) 全长 398 个氨基酸,分子量约为 ~50-70 kDa。

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描述

Serpin A3 protein is a serine protease inhibitor on chromosome 14 that affects protease targeting through tissue-specific polymorphisms. Sequence variants are associated with Alzheimer's disease, while deficiencies are associated with liver disease. Serpin A3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A3 Protein, Human (HEK293, His) is 398 a.a., with molecular weight of ~50-70 kDa.

研究背景

Serpin A3 蛋白是丝氨酸蛋白酶抑制剂家族的成员,具有丝氨酸蛋白酶抑制剂的作用。该基因位于 14 号染色体的 q 臂上,是丝氨酸蛋白酶抑制剂基因簇的一部分。Serpin A3 的多态性表现出组织特异性模式并影响蛋白酶靶向。该蛋白质序列的变异与阿尔茨海默病有关,其缺乏与肝脏疾病有关。值得注意的是,在帕金森病和慢性阻塞性肺病患者中已发现 Serpin A3 突变,强调了其在多种生理条件下的潜在作用。偏向的表达谱在肝脏 (RPKM 989.5)、胆囊 (RPKM 89.2) 和其他四种组织中显着存在,强调了其在各种生物学背景中的重要性。

生物活性

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 is 14.968 nM, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

NP_001076.2 (N26-A423)

基因 ID

12  [NCBI]

蛋白结构
N-term
Serpin A3 (N26-A423)
Accession # NP_001076.2
His
C-term
同用名
Alpha-1-Antichymotrypsin; ACT; Serpin A3; SERPINA3; AACT
氨基酸序列

NSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

分子量

Approximately 50-70 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 25 mM HEPES, 0.15M NaCl, pH 7.8.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Serpin A3 Protein, Human (HEK293, His)
目录号:
HY-P73412
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