Serpin B2 蛋白, Human

Serpin B2 inhibits urokinase-type plasminogen activator and contributes to fibrinolytic regulation. Monocyte-derived plasminogen activator inhibitor 2 (PAI-2) differs from endothelial cell-derived PAI-1, highlighting different cellular origins. Serpin B2 Protein, Human is the recombinant human-derived Serpin B2 protein, expressed by E. coli , with tag free. The total length of Serpin B2 Protein, Human is 415 a.a., with molecular weight of ~46.6 kDa.

Serpin B2 作为尿激酶型纤溶酶原激活剂的抑制剂发挥作用，有助于调节纤维蛋白溶解。值得注意的是，单核细胞来源的纤溶酶原激活剂抑制剂 2 (PAI-2) 与内皮细胞来源的 PAI-1 不同，强调了复杂的蛋白酶调节网络中的不同细胞起源和作用。Serpin B2 复杂地参与调节尿激酶型纤溶酶原激活剂的活性，反映了其在蛋白水解过程的动态相互作用中的重要性。此外，Serpin B2 与 PSMB1 相互作用，可能参与复杂的细胞途径，超出了其作为纤溶酶原激活剂抑制剂的经典作用。

Measured by its ability to inhibit uPA ( HY-P71050) cleavage of a colorimetric peptide substrate, Z-Gly-Gly-Arg-AMC (Z-GGR-AMC) (HY-P0019) that incubate at room temperature for 15 minutes. The IC₅₀ value is 14.16 nM.

Human

E. coli

Tag Free

P05120 (M1-P415)

5055  [NCBI]

MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

Approximately 46 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM Cysteine, pH 8.0, 5% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.