Serpin E1 蛋白, Mouse (HEK293, His)

Serpin E1 protein is a serine protease inhibitor that regulates TMPRSS7 and is a major inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU).It can significantly downregulate fibrinolysis and control thrombus degradation.Serpin E1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin E1 protein, expressed by HEK293 , with C-6*His labeled tag.

Serpin E1 是一种丝氨酸蛋白酶抑制剂，通过多种机制发挥其调节功能。它抑制 TMPRSS7，并作为组织型纤溶酶原激活剂 (PLAT) 和尿激酶型纤溶酶原激活剂 (PLAU) 的主要抑制剂。作为一种重要的 PLAT 抑制剂，它在下调纤维蛋白溶解和协调血栓的受控降解方面发挥着至关重要的作用。Serpin E1 作为 PLAU 抑制剂，参与细胞粘附和扩散的调节。除了作为蛋白酶抑制剂的作用外，它还充当细胞迁移的调节剂，尤其有助于刺激皮肤损伤修复过程中的角质形成细胞迁移。此外，Serpin E1 参与细胞和复制衰老，在肺中 2 型肺泡细胞的衰老中发挥作用。此外，它还参与调节牙周膜干细胞的牙骨质形成分化，并影响成牙本质细胞分化和牙本质形成。Serpin E1 与 TMPRSS7 形成异二聚体，与 VTN、PPP1CB、SORL1、LRP1 和 PLAUR 等多种蛋白质相互作用，并在不同的细胞环境中参与复杂的相互作用，强调其多方面的调节作用。

Measured by its ability to inhibit uPA cleavage of 200µM N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin(Z-GGR-AMC) that incubate at room temperature for 15minutes.The IC₅₀ value is 6.138 nM.

Materials
Assay buffer: 50 mM Tris, 0.01% (v/v) Tween? 20, pH 8.5
Serpin E1 Protein, Mouse (HEK293, His) (HY-P71133)
PLAU/uPA Protein, Human (411a.a, HEK293, His, solution) (HY-P71050)
Substrate: Z-Gly-Gly-Arg-AMC acetate (HY-P0019A)

Procedure
1. Dilute Human uPA to 2 μg/mL with assay buffer.
2. Prepare different concentrations of Human Serpin E1 with assay buffer for the following serial dilutions: 1500, 750, 375, 187.5, 125, 75, 50, 25, and 12.5 nM.
3. Equal volumes of Human Serpin E1/PAI-1 curves were mixed with diluted Human uPA at 2 μg/mL. Two controls containing only Human uPA and assay buffer were included.
4. The above mixture was incubated for 15 minutes at room temperature.
5. After incubation, the mixture was diluted 10-fold in assay buffer.
6. Dilute the substrate to 200 μM with assay buffer.
7. In a 96-well plate, add 50 μL of reaction mixture and start the reaction with 50 μL of 20 μM substrate.
8. Excitation and emission wavelengths 380 nm and 460 nm , read in kinetic mode for 5 minutes.
9. The 50% inhibitory concentration (IC₅₀) of Human Serpin E1 was derived by plotting RFU/min (or specific activity) versus concentration using a 4-PL fit.

Mouse

HEK293

C-6*His

G5E899 (T23-P402)

18787  [NCBI]

Full Length of Mature Protein

TLPLRESHTAHQATDFGVKVFQQVVQASKDRNVVFSPYGVSSVLAMLQMTTAGKTRRQIQDAMGFKVNEKGTAHALRQLSKELMGPWNKNEISTADAIFVQRDLELVQGFMPHFFKLFQTMVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVDELTRLVLVNALYFSGQWKTPFLEASTHQRLFHKSDGSTVSVPMMAQSNKFNYTEFTTPDGLEYDVVELPYQGDTLSMFIAAPFEKDVHLSALTNILDAELIRQWKGNMTRLPRLLILPKFSLETEVDLRGPLEKLGMPDMFSATLADFTSLSDQEQLSVAQALQKVRIEVNESGTVASSSTAFVISARMAPTEMVIDRSFLFVVRHNPTETILFMGQVMEP

Approximately 48.54 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.