SETD7 蛋白, Human (His)

The SETD7 protein is a histone methyltransferase that specifically monomethylates H3K4, a mark associated with transcriptional activation. Its role in gene activation extends to the collagenase and insulin genes, specifically at the insulin promoter when IPF1/PDX-1 is recruited. SETD7 Protein, Human (His) is the recombinant human-derived SETD7 protein, expressed by E. coli , with N-His labeled tag. The total length of SETD7 Protein, Human (His) is 365 a.a., with molecular weight of 47-50 kDa.

SETD7 蛋白是一种组蛋白甲基转移酶，在组蛋白 H3 的“Lys-4”单甲基化方面表现出特异性。这种甲基化事件是与表观遗传转录激活相关的独特标记。SETD7 在胶原酶或胰岛素等基因的转录激活中发挥着关键作用。它被 IPF1/PDX-1 招募到胰岛素启动子上，导致转录激活。除了组蛋白之外，SETD7 对非组蛋白（包括 CGAS、p53/TP53、TAF10 和可能的 TAF7）表现出甲基转移酶活性，识别并结合底物蛋白中的 [KR]-[STA]-K 基序。TAF10 的“Lys-189”单甲基化增强了其对 RNA 聚合酶 II 的亲和力。此外，SETD7 使 p53/TP53 的“Lys-372”单甲基化，稳定蛋白质并放大 p53/TP53 介导的转录激活。此外，它使 CGAS 的“Lys-491”单甲基化，促进 SGF29 和 CGAS 之间的相互作用（通过相似性）。

Human

E. coli

N-His

Q8WTS6/NP_085151.1 (D2-K366)

80854  [NCBI]

DSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

47-50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.