2. 重组蛋白
  3. CAR-T related Proteins CD Antigens Receptor Proteins Biotinylated Proteins
  4. B Cell CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins Siglec
  5. Siglec-2/CD22
  6. Siglec-2/CD22 蛋白, Mouse (Biotinylated, HEK293, His)

Siglec-2/CD22 蛋白, Mouse (Biotinylated, HEK293, His)

目录号: HY-P701288
产品使用指南

Siglec-2/CD22 是一种 B 细胞表面受体，介导 B 细胞相互作用并参与淋巴组织内的 B 细胞定位。它选择性结合 α-2,6 连接的唾液酸，包括 CD45，其唾液酸识别位点可能受顺式相互作用调节。在免疫反应中，配体诱导的酪氨酸磷酸化表明在 B 细胞抗原受体信号传导调节中发挥作用。它通过与 Src 家族激酶相互作用并通过其 SH2 结构域招募细胞质磷酸酶来参与正向和抑制性调节。相互作用包括磷酸化后的 LYN、SYK、PIK3R1/PIK3R2、PLCG1、SHC1、INPP5D 和 GRB2，以及磷酸化后的 PTPN6/SHP-1。Siglec-2/CD22 蛋白, Mouse (Biotinylated, HEK293, His) 是重组的 Siglec-2/CD22 蛋白，由 HEK293 表达，带有 His 标签。Siglec-2/CD22 蛋白, Mouse (Biotinylated, HEK293, His) 全长 681 个氨基酸，分子量为 90-110 kDa。

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描述

Siglec-2/CD22, a B-cell surface receptor, mediates B-cell interactions and participates in B-cell localization within lymphoid tissues. It selectively binds alpha-2,6-linked sialic acid, including CD45, with its sialic acid recognition site potentially regulated by cis interactions. In the immune response, ligand-induced tyrosine phosphorylation suggests a role in B-cell antigen receptor signaling modulation. It engages in both positive and inhibitory regulation by interacting with Src family kinases and recruiting cytoplasmic phosphatases via their SH2 domains. Interactions include LYN, SYK, PIK3R1/PIK3R2, PLCG1, SHC1, INPP5D, and GRB2 upon phosphorylation, as well as PTPN6/SHP-1 upon phosphorylation. Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with His labeled tag. The total length of Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is 681 a.a., with molecular weight of 90-110 kDa.
研究背景

Siglec-2/CD22 蛋白充当 B 细胞相互作用的介质，并可能参与 B 细胞在淋巴组织内的定位。该蛋白选择性地结合唾液酸化糖蛋白，优先结合 α-2,6 连接的唾液酸，其结合伴侣之一是 CD45。唾液酸的识别位点可以通过与同一细胞表面上的唾液酸的顺式相互作用来掩蔽。在免疫反应期间配体诱导的酪氨酸磷酸化后，Siglec-2/CD22 参与 B 细胞抗原受体信号传导的调节。它在信号传导中发挥双重作用，积极调节与 Src 家族酪氨酸激酶的相互作用，并通过其 SH2 结构域招募细胞质磷酸酶，潜在地充当抑制性受体。这种招募通过信号分子的去磷酸化来阻断信号转导。在分子相互作用中，Siglec-2/CD22 在磷酸化后与 LYN、SYK、PIK3R1/PIK3R2、PLCG1、SHC1、INPP5D 和 GRB2 等蛋白质结合，表明其参与调节 B 细胞反应的复杂信号网络。
种属

Mouse
表达系统

HEK293
标签

His
蛋白编号

P35329-1 (S22-P702)
基因 ID

12483
同用名
CD22; SIGLEC2; BL-CAM; SIGLEC-2; Siglec2; SIGLEC2FLJ22814
分子量

90-110 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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