1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a 蛋白, Human (HEK293, Fc)

SIRP alpha/CD172a 蛋白, Human (HEK293, Fc)

目录号: HY-P7406
COA 产品使用指南

SIRP alpha/CD172a 蛋白, Human (P78324, HEK293, Fc)在 HEK293 细胞中产生,是一种含有 572 个氨基酸的多肽链,具有 C-端人 IgG1 Fc 片段。SIRPα 是一种单体,约 90kDa 的 I 型跨膜糖蛋白,属于免疫球蛋白超家族的 SIRP/SHPS (CD172) 家族。

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规格 价格 是否有货 数量
10 μg ¥890 In-stock
50 μg ¥2500 In-stock
100 μg   询价  

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描述

SIRP alpha/CD172a Protein, Human (P78324, HEK293, Fc) produced in HEK293 cells is a polypeptide chain containing 572 amino acids with the C-termimal human IgG1 Fc fragment. Signal regulatory protein alpha (SIRPα, designated CD172a) is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily.

研究背景

信号调节蛋白 α (SIRPα) 是一种糖蛋白受体,通过酪氨酸磷酸酶 SHP-1 和 SHP-2 募集并发出信号。SIRPα 的胞质结构域包含四个基于免疫受体酪氨酸的抑制基序 (ITIM),这些基序在磷酸化后募集并激活含有 SH2 结构域的磷酸酪氨酸磷酸酶 (PTPase) SHP-1 和 SHP-2。在巨噬细胞中,SIRPα 可以负向调节宿主细胞的吞噬作用和肿瘤坏死因子 α 的产生。SIRPα 连接通过 JAK/STAT 和 PI3-K/Rac1/NOX/H2O2 信号通路的协同作用诱导巨噬细胞产生 NO。它提出 SIRPα 可能充当抑制性受体[1]

生物活性

Immobilized CD47, His, Human at 2.0 μg/mL (100 µl/well), can bind SIRPα, hFc, Human with a linear range of 0.25-185.0 ng/mL.

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

P78324 (E31-R370)

基因 ID
蛋白结构
N-term
CD172a (E31-R370)
Accession # P78324
hFc
C-term
中文名
重组人信号调节蛋白α, Fc 嵌合体 (HEK293 细胞表达)
同用名
rHuSignal regulatory protein α, Fc Chimera; CD172a; SHPS-1; BIT; MYD1
氨基酸序列

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

分子量

85-100 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS or PBS, 5% trehalose and mannitol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SIRP alpha/CD172a Protein, Human (HEK293, Fc)
目录号:
HY-P7406
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