SLPI 蛋白, Human (107a.a, sf9, His, solution)

SLPI Protein, an acid-stable proteinase inhibitor, demonstrates strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G in various studies. It crucially modulates inflammatory and immune responses post-bacterial infection and L.major infection. SLPI down-regulates responses to bacterial lipopolysaccharide (LPS), regulates NF-kappa-B activation, impacting inflammatory responses. It exhibits antimicrobial activity against mycobacteria, contributing to resistance against M.tuberculosis. Essential for normal resistance to L.major infection, SLPI plays a vital role in wound healing, preventing tissue damage by regulating protease activity. Collaborating with ELANE, SLPI is necessary for normal bone marrow myeloid cell differentiation and proliferation, interacting with GRN to protect progranulin from proteolysis. SLPI Protein, Human (107a.a, sf9, His, solution) is the recombinant human-derived SLPI protein, expressed by Sf9 insect cells, with N-6*His labeled tag. The total length of SLPI Protein, Human (107a.a, sf9, His, solution) is 107 a.a., with molecular weight of ~19 kDa.

SLPI 是一种酸稳定的蛋白酶抑制剂，对胰蛋白酶、胰凝乳蛋白酶、弹性蛋白酶和组织蛋白酶 G 表现出强大的亲和力，正如各种研究所证明的那样。它在调节细菌感染以及细胞内寄生虫 L.major 感染后的炎症和免疫反应中发挥着至关重要的作用。此外，SLPI 有助于下调对细菌脂多糖 (LPS) 的反应（通过相似性），并参与调节 NF-κ-B 的激活，从而影响炎症反应。值得注意的是，SLPI 对分枝杆菌具有抗菌活性，但对沙门氏菌没有抗菌活性，有助于对结核分枝杆菌感染的正常抵抗力。它对于正常抵抗 L.major 感染也至关重要，并且在伤口愈合中发挥着关键作用，可能是通过调节蛋白酶活性来防止组织损伤（通过相似性）。此外，与 ELANE 合作，SLPI 是骨髓细胞正常分化和增殖所必需的，并且它与 GRN 相互作用，保护颗粒体蛋白前体免受蛋白水解。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK (Dnp)-NH2 and the IC₅₀ value is <1 Nm.

Human

Sf9 insect cells

N-6*His

P03973 (S26-A132)

6590  [NCBI]

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.