SPARC 蛋白, Human (HEK293, His)

SPARC proteins are essential for lipid transport and are involved in the production, transformation, and clearance of plasma lipoproteins. It interacts with different particles, shows a preference for HDL, and binds to cellular receptors such as LDLR and VLDLR to promote the uptake of APOE-containing lipoproteins. SPARC Protein, Human (HEK293, His) is the recombinant human-derived SPARC protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SPARC Protein, Human (HEK293, His) is 286 a.a., with molecular weight of ~38.0 kDa.

APOE 是一种重要的蛋白质，参与通过血浆和间质液在器官之间运输脂质。它在血浆脂蛋白的产生、转化和清除中起着至关重要的作用。APOE 与各种脂蛋白颗粒相互作用，包括乳糜微粒、乳糜微粒残余物、VLDL 和 IDL，特别是 HDL。它还与多种细胞受体结合，例如 LDLR 和 VLDLR，促进细胞摄取含有 APOE 的脂蛋白。此外，APOE 具有肝素结合活性，可与细胞表面的硫酸乙酰肝素蛋白聚糖结合，有助于捕获和受体介导的含 APOE 脂蛋白的摄取。此外，APOE 形成同源四聚体，并可能在 HDL 生物合成中与 ABCA1 相互作用。它还可以与 APP/A4 淀粉样蛋白-β 肽、MAPT、MAP2、分泌的 SORL1 和 PMEL 相互作用，实现各种生理过程。

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is typically 2.503 μg/mL, corresponding to a specific activity is 400 U/mg.

Human

HEK293

C-6*His

P09486 (A18-I303 )

6678  [NCBI]

APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI

Approximately 38.0-46 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.