ST2/IL1RL1 蛋白, Mouse (HEK293, His)

The ST2/IL1RL1 protein serves as a receptor for IL-33 and plays a crucial role in immunity and response to environmental stress through IL1RAP. It recruits MYD88, IRAK1/4 and TRAF6 upon stimulation, leading to MAPK phosphorylation. ST2/IL1RL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ST2/IL1RL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ST2/IL1RL1 Protein, Mouse (HEK293, His) is 311 a.a., with molecular weight of 50-70 kDa.

ST2/IL1RL1 是白细胞介素 33 (IL-33) 的受体，与其辅助受体 IL1RAP 结合，在先天免疫和适应性免疫中发挥着至关重要的作用，以维持组织稳态并响应环境压力。受到刺激后，它会招募 MYD88、IRAK1、IRAK4 和 TRAF6，导致各种 MAPK 磷酸化，包括 MAPK3/ERK1、MAPK1/ERK2、MAPK14 和 MAPK8。该受体与辅助 T 细胞功能有关，并且在组织损伤的情况下，诱导 UCP2 依赖性线粒体重连。这种重新布线减少了活性氧的产生，保持了克雷布斯循环的完整性。这种保存对于衣康酸的持续产生以及随后的消炎替代激活巨噬细胞的 GATA3 依赖性分化至关重要。此外，ST2/IL1RL1 在抑制 IL-33 信号传导中发挥作用。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 34.04 ng/mL in the presence of 20 ng/mL recombinant mouse IL-33, corresponding to a specific activity is 2.51×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 34.04 ng/mL in the presence of 20 ng/mL recombinant mouse IL-33, corresponding to a specific activity is 2.51×10⁵ units/mg.

Mouse

HEK293

C-6*His

P14719-2 (S27-A337)

17082  [NCBI]

SKSSWGLENEALIVRCPQRGRSTYPVEWYYSDTNESIPTQKRNRIFVSRDRLKFLPARVEDSGIYACVIRSPNLNKTGYLNVTIHKKPPSCNIPDYLMYSTVRGSDKNFKITCPTIDLYNWTAPVQWFKNCKALQEPRFRAHRSYLFIDNVTHDDEGDYTCQFTHAENGTNYIVTATRSFTVEEKGFSMFPVITNPPYNHTMEVEIGKPASIACSACFGKGSHFLADVLWQINKTVVGNFGEARIQEEEGRNESSSNDMDCLTSVLRITGVTEKDLSLEYDCLALNLHGMIRHTIRLRRKQPSKECPSHIA

50-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.