TFPI2 蛋白, Mouse (HEK293, His)

TFPI2 (tissue factor pathway inhibitor 2) is a key regulator of matrix remodeling and has inhibitory control effects on trypsin, plasmin and factor VIIa/tissue factor. It has a weak effect on factor Xa and has no effect on thrombin. Its complex interactions extend to the formation of molecular complexes with ABCB1 and PPP2R3C, resulting in dephosphorylation of ABCB1. TFPI2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TFPI2 protein, expressed by HEK293 , with C-His labeled tag. The total length of TFPI2 Protein, Mouse (HEK293, His) is 208 a.a., with molecular weight of 35-50 kDa.

TFPI2（组织因子途径抑制剂 2）是纤溶酶精心策划的复杂基质重塑网络中的关键调节因子。其抑制能力延伸至胰蛋白酶、纤溶酶和因子 VIIa/组织因子，对因子 Xa 的影响较弱，同时对凝血酶保持惰性。除了直接抑制作用之外，TFPI2 还参与复杂的相互作用，与 ABCB1 和 PPP2R3C 形成分子联盟。这种复杂的形成导致 ABCB1 去磷酸化，突显 TFPI2 参与超出其主要抑制功能的动态细胞过程。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC₅₀ value is 1.68 nM, as measured under the described conditions.

Mouse

HEK293

C-His

O35536 (L23-S230)

21789  [NCBI]

LTSVSAQGNNLEICLLPLDAGPCQALIPKFYYDRDQQKCRRFNYGGCLGNANNFHSRDLCQQTCGSIEKVPPVCRSELKTYPCDKPNIRFFFNLNTMTCEPLRPGLCSRTINVFSEEATCKGLCEPRKHIPSFCSSPKDEGLCSANVTRFYFNSRNKTCETFTYTGCGGNENNFYYLDACHRACVKGWKKPKRWKIGDFLPRFWKHLS

35-50 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 150 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.