TFRC 蛋白, Mouse (HEK293, His)

TFRC is a key protein in cellular iron uptake, coordinating receptor-mediated endocytosis of the ligand transferrin into specialized endosomes, where endosomal acidification triggers iron release. TFRC actively regulates T and B cell proliferation through iron uptake. TFRC Protein, Mouse (HEK293, His) is the recombinant mouse-derived TFRC protein, expressed by HEK293 , with N-8*His labeled tag. The total length of TFRC Protein, Mouse (HEK293, His) is 675 a.a., with molecular weight of ~90 kDa.

TFRC 是细胞铁吸收的关键蛋白，协调受体介导的配体转铁蛋白的内吞作用进入专门的内体，内体酸化触发铁释放。脱铁转铁蛋白-受体复合物在pH值恢复到中性的同时循环到细胞表面，导致脱铁转铁蛋白对其受体的亲和力丧失。TFRC 对红细胞和神经系统发育至关重要，通过铁的摄取积极调节 T 细胞和 B 细胞增殖。作为脂质传感器，TFRC 通过调节 JNK 通路来调节线粒体融合。低膳食硬脂酸水平会诱导 JNK 通路激活，导致 HUWE1 介导的泛素化和线粒体融合蛋白 MFN2 的降解，从而抑制线粒体融合。相反，高硬脂酸水平会使 TFRC 硬脂酰化，抑制 JNK 通路激活并防止 MFN2 降解。TFRC 还可以作为具有二硫键的同型二聚体，每个亚基结合一个转铁蛋白分子，并与 SH3BP4 和 STEAP3 相互作用，后者促进红系前体细胞中的 TFRC 内吞作用。

Mouse

HEK293

N-8*His

Q62351 (C89-F763)

22042  [NCBI]

CKRVEQKEECVKLAETEETDKSETMETEDVPTSSRLYWADLKTLLSEKLNSIEFADTIKQLSQNTYTPREAGSQKDESLAYYIENQFHEFKFSKVWRDEHYVKIQVKSSIGQNMVTIVQSNGNLDPVESPEGYVAFSKPTEVSGKLVHANFGTKKDFEELSYSVNGSLVIVRAGEITFAEKVANAQSFNAIGVLIYMDKNKFPVVEADLALFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAAAEKLFGKMEGSCPARWNIDSSCKLELSQNQNVKLIVKNVLKERRILNIFGVIKGYEEPDRYVVVGAQRDALGAGVAAKSSVGTGLLLKLAQVFSDMISKDGFRPSRSIIFASWTAGDFGAVGATEWLEGYLSSLHLKAFTYINLDKVVLGTSNFKVSASPLLYTLMGKIMQDVKHPVDGKSLYRDSNWISKVEKLSFDNAAYPFLAYSGIPAVSFCFCEDADYPYLGTRLDTYEALTQKVPQLNQMVRTAAEVAGQLIIKLTHDVELNLDYEMYNSKLLSFMKDLNQFKTDIRDMGLSLQWLYSARGDYFRATSRLTTDFHNAEKTNRFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVENLKLRQKNITAFNETLFRNQLALATWTIQGVANALSGDIWNIDNEF

Approximately 90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.