TIGIT 蛋白, Human (HEK293, mFc)

The TIGIT protein plays a key role in immune regulation and binds with high affinity to the poliovirus receptor (PVR). This interaction induces an immunosuppressive environment by increasing IL10 secretion and decreasing IL12B secretion. TIGIT Protein, Human (HEK293, mFc) is the recombinant human-derived TIGIT protein, expressed by HEK293 , with C-mFc labeled tag. The total length of TIGIT Protein, Human (HEK293, mFc) is 120 a.a., with molecular weight of 40-50 kDa.

TIGIT 蛋白在免疫调节中发挥着关键作用，与脊髓灰质炎病毒受体 (PVR) 表现出高亲和力结合。这种相互作用导致 IL10 分泌增加和 IL12B 分泌减少，从而形成免疫抑制环境。TIGIT通过抑制T细胞活化和促进成熟免疫调节树突状细胞的生成进一步发挥其免疫调节作用。在结构上，TIGIT 形成顺式同二聚体，以高亲和力与 PVR 结合，从而产生包含两个 TIGIT 和两个 PVR 分子的异四聚体组装体。此外，TIGIT 与 NECTIN2 和 NECTIN3 的结合亲和力较低，强调了其多种分子相互作用的能力。TIGIT 的多方面功能和结合亲和力凸显了其在免疫调节中的关键作用及其作为调节免疫反应的治疗靶点的潜力。

Human

HEK293

C-mFc

Q495A1 (M22-P141)

201633  [NCBI]

MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP

40-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.