1. 重组蛋白
  2. Immune Checkpoint Proteins
  3. Inhibitory checkpoint molecules
  4. TIGIT Protein
  5. TIGIT 蛋白, Human (HEK293, Fc)

TIGIT 蛋白, Human (HEK293, Fc)

目录号: HY-P7414
COA 产品使用指南

TIGIT 蛋白, Human (HEK293, Fc)是在 HEK293 细胞中产生的含有352个氨基酸的多肽链,带有C-端的人 IgG1 Fc 片段。T-cell immunoreceptor with Ig and ITIM domains (TIGIT),也被称为 V-set 和免疫球蛋白结构域的蛋白9 (VSIG9)、V-set 和跨膜结构域的蛋白3 (VSTM3)。它属于单通道 I 型膜蛋白,包含免疫球蛋白可变结构域、跨膜结构域和免疫受体酪氨酸抑制基序 (ITIM)。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥3200 In-stock
100 μg   询价  
250 μg   询价  

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描述

TIGIT Protein, Human (HEK293, Fc) produced in HEK293 cells is a polypeptide chain containing 352 amino acids with the C-termimal human IgG1 Fc fragment. T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3). It belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM).

研究背景

T cell Ig and ITIM domain (TIGIT) is a transmembrane glycoprotein belonging to a poliovirus receptor family of type 1 proteins that also binds to CD155 and CD112 ligands. TIGIT is expressed on peripheral memory and regulatory CD4+ T cells and NK cells and is upregulated following activation on naive CD4+ T cells. CD155 is a high-affinity receptor for TIGIT expressed on monocytes and CD11c+ human dendritic cells (DCs). TIGIT contains an Ig-like V-type domain and an ITIM in its cytoplasmic domain, suggesting that receptor occupancy may trigger a negative signaling event[1].

生物活性

5 µg/mL (100 µL/well) of immoblized recombinant human CD155-Fc can bind human Biotin-TIGIT-Fc with a linear range of 6.1-48.83 ng/mL.

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q495A1 (M22-P141)

基因 ID

201633  [NCBI]

中文名
重组人 TIGIT,FC 嵌合体 (HEK293 细胞表达)
同用名
rHuTIGIT, Fc Chimera; VSIG9; VSTM3; T Cell Immunoreceptor with Ig and ITIM Domains
氨基酸序列

MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP

分子量

50-55 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

TIGIT Protein, Human (HEK293, Fc) 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIGIT Protein, Human (HEK293, Fc)
目录号:
HY-P7414
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