TIMP-4 蛋白, Human (HEK293, His)

TIMP-4, a member of the tissue inhibitor of metalloproteinases family, forms complexes with metalloproteinases, including collagenases, and exerts irreversible inactivation by binding to their catalytic zinc cofactor. Its inhibitory effect is recognized on a range of metalloproteases, particularly MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. TIMP-4 Protein, Human (HEK293, His) is the recombinant human-derived TIMP-4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TIMP-4 Protein, Human (HEK293, His) is 195 a.a., with molecular weight of ~24.0 kDa.

金属蛋白酶组织抑制剂 4 (TIMP-4) 是一种与金属蛋白酶（包括胶原酶）形成复合物的蛋白质，通过与其催化锌辅因子结合导致其不可逆失活。TIMP-4 具有广谱抑制活性，已知可作用于各种基质金属蛋白酶 (MMP)，例如 MMP-1、MMP-2、MMP-3、MMP-7 和 MMP-9。通过调节这些 MMP 的活性，TIMP-4 在调节细胞外基质重塑方面发挥着至关重要的作用，从而影响组织发育、维护和修复等过程。TIMP-4 与多种 MMP 相互作用并抑制多种 MMP 的能力凸显了其在维持蛋白水解活性平衡中的重要性，并强调了其在各种生理和病理环境中的潜在意义。（参考：提供的资料）

Human

HEK293

C-6*His

Q99727 (C30-P224)

7079  [NCBI]

CSCAPAHPQQHICHSALVIRAKISSEKVVPASADPADTEKMLRYEIKQIKMFKGFEKVKDVQYIYTPFDSSLCGVKLEANSQKQYLLTGQVLSDGKVFIHLCNYIEPWEDLSLVQRESLNHHYHLNCGCQITTCYTVPCTISAPNECLWTDWLLERKLYGYQAQHYVCMKHVDGTCSWYRGHLPLRKEFVDIVQP

Approximately 24.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.