TLR2 蛋白, Human (sf9, His)

TLR2 is a protein that plays a critical role in recognizing and responding to various bacterial and mycobacterial pathogens. TLR2 Protein, Human (sf9, His) is the recombinant human-derived TLR2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag. The total length of TLR2 Protein, Human (sf9, His) is 567 a.a., with molecular weight of ~65.5 kDa.

TLR2 蛋白与 LY96 协同协调针对细菌脂蛋白和各种微生物细胞壁成分的先天免疫反应。此外，TLR2 与 TLR1 或 TLR6 形成功能复合物以识别细菌脂肽。通过涉及 MYD88 和 TRAF6 的信号通路，TLR2 激活导致 NF-kappa-B 激活、细胞因子分泌和炎症反应的启动。该受体在识别特定配体方面发挥着关键作用，例如支原体巨噬细胞激活脂肽-2kD (MALP-2) 和伯氏疏螺旋体外表面蛋白 A 脂蛋白 (OspA-L)，通常与 TLR6 协同作用。TLR2 与其他受体（包括 CD14 和 CD36）进行复杂的相互作用，形成激活簇，在通过脂筏依赖性途径引导至高尔基体之前触发细胞表面的信号传导事件。此外，TLR2 对于结核分枝杆菌的正常摄取至关重要，其相互作用延伸至各种调节蛋白，例如 MYD88、TICAM1 和 TIRAP。

Human

Sf9 insect cells

C-10*His

O60603 (E21-R587)

7097  [NCBI]

ESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDRVIDPGKVETLTIRRLHIPRFYLFYDLSTLYSLTERVKRITVENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACEDAWPSLQTLILRQNHLASLEKTGETLLTLKNLTNIDISKNSFHSMPETCQWPEKMKYLNLSSTRIHSVTGCIPKTLEILDVSNNNLNLFSLNLPQLKELYISRNKLMTLPDASLLPMLLVLKISRNAITTFSKEQLDSFHTLKTLEAGGNNFICSCEFLSFTQEQQALAKVLIDWPANYLCDSPSHVRGQQVQDVRLSVSECHR

Approximately 65.5 kDa

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.