TLR4 蛋白, Human (sf9, His, Solution)

TLR4 is a transmembrane receptor that recognizes PAMPs and DAMPs and cooperates with LY96 for LPS detection. TLR4 activates MYD88-dependent and independent signaling pathways and participates in TIRAP, TRAF6 and CD14-mediated endocytosis. TLR4 Protein, Human (sf9, His, Solution) is the recombinant human-derived TLR4 protein, expressed by Sf9 insect cells, with C-His labeled tag.

TLR4 是一种跨膜受体，充当模式识别受体，检测病原体和损伤相关的分子模式（PAMP 和 DAMP）以启动先天免疫反应。在质膜上，它与 LY96 合作识别细菌脂多糖 (LPS)，从而引发免疫反应。此外，TLR4 参与由游离脂肪酸和 Ni(2+) 诱导的不依赖于 LPS 的炎症反应。该受体通过涉及 MYD88、TIRAP 和 TRAF6 的信号通路发挥作用，导致 NF-kappa-B 激活和细胞因子分泌。此外，CD14 介导的 TLR4 通过胞吞作用内化，通过 TICAM1-TBK1-IRF3 轴启动不依赖于 MYD88 的信号通路，从而产生 I 型干扰素。TLR4 激活还会导致 NLRP3 炎症小体的激活以及自噬和 NF-kappa-B 信号级联之间的正反馈回路。与 TLR6 复合，TLR4 促进单核细胞/巨噬细胞的炎症，配体结合触发内化和炎症反应。TLR4 的多种相互作用，包括与 LY96、MYD88、TIRAP、TICAM2、NOX4、CNPY3、HSP90B1、CD36、WDFY1、SMPDL3B、CEACAM1、RFTN1、SCIMP、TRAF3IP3 和 TREM1 的相互作用，进一步凸显了其在协调免疫反应和细胞信号传导中的关键作用。

Immobilized Recombinant Human TLR4 / CD284 Protein (His Tag) at 1 μg/mL (100 μl/well) on His Tag Antibody, Mouse MAb precoated (5 μg/mL, 100 μL/well) can bind Anti-TLR4 Antibody, the EC₅₀ is 3.784-15 ng/mL.

Human

Sf9 insect cells

C-His

O00206-1 (E24-K631)

7099

Partial

ESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNK

Approximately 68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 87% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 6.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.