TLR4 蛋白, Human (sf9, His)

TLR4 is a transmembrane receptor that recognizes PAMPs and DAMPs and cooperates with LY96 for LPS detection. TLR4 activates MYD88-dependent and independent signaling pathways and participates in TIRAP, TRAF6 and CD14-mediated endocytosis. TLR4 Protein, Human (sf9, His) is the recombinant human-derived TLR4 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of TLR4 Protein, Human (sf9, His) is 608 a.a., with molecular weight of ~68 kDa.

TLR4 是一种跨膜受体，充当模式识别受体，检测病原体和损伤相关的分子模式（PAMP 和 DAMP）以启动先天免疫反应。在质膜上，它与 LY96 合作识别细菌脂多糖 (LPS)，从而引发免疫反应。此外，TLR4 参与由游离脂肪酸和 Ni(2+) 诱导的不依赖于 LPS 的炎症反应。该受体通过涉及 MYD88、TIRAP 和 TRAF6 的信号通路发挥作用，导致 NF-kappa-B 激活和细胞因子分泌。此外，CD14 介导的 TLR4 通过胞吞作用内化，通过 TICAM1-TBK1-IRF3 轴启动不依赖于 MYD88 的信号通路，从而产生 I 型干扰素。TLR4 激活还会导致 NLRP3 炎症小体的激活以及自噬和 NF-kappa-B 信号级联之间的正反馈回路。与 TLR6 复合，TLR4 促进单核细胞/巨噬细胞的炎症，配体结合触发内化和炎症反应。TLR4 的多种相互作用，包括与 LY96、MYD88、TIRAP、TICAM2、NOX4、CNPY3、HSP90B1、CD36、WDFY1、SMPDL3B、CEACAM1、RFTN1、SCIMP、TRAF3IP3 和 TREM1 的相互作用，进一步凸显了其在协调免疫反应和细胞信号传导中的关键作用。

Human

Sf9 insect cells

C-His

O00206 (E24-K631)

7099  [NCBI]

Approximately 68 kDa

Greater than 87% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 6.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.