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  5. TMPRSS2 蛋白, Human (R255Q, HEK293, His)

TMPRSS2 蛋白, Human (R255Q, HEK293, His)

目录号: HY-P72044
COA 产品使用指南

TMPRSS2 蛋白是一种质膜锚定的丝氨酸蛋白酶,在前列腺生理学、癌症转移、疼痛调节和病毒感染中发挥着关键作用。其裂解活性影响涉及前列腺功能的蛋白水解级联,而雄激素诱导的激活有助于癌症进展。TMPRSS2 蛋白, Human (R255Q, HEK293, His) 是重组的 TMPRSS2 蛋白,由 HEK293 表达,带有 N-10*His 标签。 和 R255Q,,, 突变。TMPRSS2 蛋白, Human (R255Q, HEK293, His) 全长 387 个氨基酸,分子量约为 ~46.71 kDa。

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规格 价格 是否有货 数量
10 μg ¥2800 In-stock
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100 μg   询价  

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描述

The TMPRSS2 protein is a plasma membrane-anchored serine protease that plays critical roles in prostate physiology, cancer metastasis, pain modulation, and viral infection. Its lytic activity affects proteolytic cascades involved in prostate function, and androgen-induced activation contributes to cancer progression. TMPRSS2 Protein, Human (R255Q, HEK293, His) is the recombinant human-derived TMPRSS2 protein, expressed by HEK293 , with N-10*His labeled tag and R255Q, , , , mutation. The total length of TMPRSS2 Protein, Human (R255Q, HEK293, His) is 387 a.a., with molecular weight of ~46.71 kDa.

研究背景

TMPRSS2 蛋白是一种质膜锚定的丝氨酸蛋白酶,在各种生理和病理过程中发挥着独特的作用。TMPRSS2 以其精氨酸残基的裂解活性而闻名,它在对前列腺正常生理功能至关重要的蛋白水解级联中发挥着关键作用。在前列腺癌中,雄激素诱导的 TMPRSS2 激活导致前肝细胞生长因子/HGF、蛋白酶激活受体 2/F2RL1 和 matriptase/ST14 等底物裂解,促进细胞外基质破坏和转移。此外,TMPRSS2 通过激活三叉神经元来调节疼痛敏感性,影响自发性疼痛和机械性异常性疼痛。在微生物感染领域,TMPRSS2 通过两种独立的机制,在促进人类冠状病毒 SARS-CoV 和 SARS-CoV-2 的感染中发挥着关键作用:ACE2 受体的蛋白水解裂解,促进病毒摄取,以及冠状病毒刺突的裂解糖蛋白,激活糖蛋白进入宿主细胞。这种蛋白酶对于甲型流感病毒的传播和发病机制也至关重要,参与血凝素 (HA) 蛋白的蛋白水解裂解和激活,而血凝素 (HA) 蛋白是病毒感染性所必需的。TMPRSS2 的多种功能强调了其在正常生理过程和疾病发病机制中的重要性。

生物活性

Data is not available.

种属

Human

表达系统

HEK293

标签

N-10*His

蛋白编号

O15393-1 (W106-G492, R255Q)

基因 ID
蛋白结构
N-term
10*His
TMPRSS2 (W106-G492, R255Q)
Accession # O15393-1
C-term
同用名
Serine protease 10
氨基酸序列

WKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSQIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG

分子量

Approximately 46.71 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE
性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 10 mM Tris-HCl, 150 mM NaCl, 50% glycerin, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

TMPRSS2 Protein, Human (R255Q, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TMPRSS2 Protein, Human (R255Q, HEK293, His)
目录号:
HY-P72044
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