Toll-like receptor 8/TLR8 蛋白, Human (P.pastoris, His)

The TLR8 protein is an endosomal receptor critical for innate and adaptive immunity. Toll-like receptor 8/TLR8 Protein, Human (P.pastoris, His) is the recombinant human-derived Toll-like receptor 8/TLR8 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Toll-like receptor 8/TLR8 Protein, Human (P.pastoris, His) is 801 a.a., with molecular weight of ~93.5 kDa.

Toll 样受体 8 (TLR8) 蛋白是一种对先天性和适应性免疫至关重要的内体受体，通过识别微生物特有的 RNA 降解产物（最初由 RNASET2 加工），在协调宿主针对病原体的免疫反应中发挥着至关重要的作用。值得注意的是，TLR8 擅长识别源自各种病毒的富含 GU 的单链 RNA（GU-rich RNA），包括 SARS-CoV-2、SARS-CoV-1 和 HIV-1。与激动剂结合后，TLR8 发生二聚化，促进 Toll/Interleukin-1 受体 (TIR) 结构域的直接接触，从而通过同型相互作用招募下游接头 MYD88。这引发了涉及 IRAK4、IRAK1、TRAF6 和 TRAF3 的 Myddosome 信号复合物的形成，最终激活下游转录因子 NF-kappa-B 和 IRF7，诱导促炎细胞因子和干扰素。TLR8 的激活尤其是由含有足够数量尿苷的 RNA 触发的，这强调了其在致病性 RNA 识别和免疫反应启动方面的特异性。

Human

P. pastoris

N-6*His

Q9NR97 (E27-T827)

51311  [NCBI]

EENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVT

Approximately 93.5kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.2 μm filtered solution in 20 mM Tris-HC1, 0.5 M NaCl, 3% Trehalose, pH 8.0.

<3 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.