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  3. TPBG/5T4 蛋白, Mouse (HEK293, His)

TPBG/5T4 蛋白, Mouse (HEK293, His)

目录号: HY-P70121
产品使用指南

TPBG/5T4 蛋白可作为 Wnt/β-连环蛋白信号传导的抑制剂,可能是通过与 LRP6 间接相互作用并阻碍 Wnt3a 依赖性 LRP6 内化而发挥作用。这表明 TPBG/5T4 在调节复杂的 Wnt/β-catenin 信号通路并影响与该通路相关的细胞反应中发挥着关键作用。TPBG/5T4 蛋白, Mouse (HEK293, His) 是重组的 TPBG/5T4 蛋白,由 HEK293 表达,带有 C-6*His 标签。TPBG/5T4 蛋白, Mouse (HEK293, His) 全长 330 个氨基酸,分子量为 50-70 kDa。

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描述

TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization. This suggests that TPBG/5T4 plays a key role in regulating the complex Wnt/β-catenin signaling pathway and influencing cellular responses related to this pathway. TPBG/5T4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TPBG/5T4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TPBG/5T4 Protein, Mouse (HEK293, His) is 330 a.a., with molecular weight of 50-70 kDa.

研究背景

TPBG/5T4 蛋白似乎作为 Wnt/β-连环蛋白信号传导的抑制剂发挥作用,可能通过与 LRP6 间接相互作用并阻碍 Wnt3a 依赖性 LRP6 内化来发挥其抑制作用。这表明 TPBG/5T4 可能在调节复杂的 Wnt/β-catenin 信号通路、影响与该通路相关的细胞反应和功能中发挥关键作用。对 TPBG/5T4 和 LRP6 之间相互作用的具体分子机制及其对 Wnt3a 依赖性 LRP6 内化的影响进行全面研究,可以为 TPBG/5T4 在 Wnt/β- 调节中的功能意义提供有价值的见解。连环蛋白信号传导及其对细胞过程的潜在影响。

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9Z0L0 (S32-S361)

基因 ID

21983  [NCBI]

蛋白结构
N-term
TLR8 (S32-S361)
Accession # Q9Z0L0
6*His
C-term
同用名
rMuTrophoblast glycoprotein/5T4, His; TPBG; 5T4 Protein, Human; 5T4AG Protein, Human; M6P1 Protein, Human; WAIF1 Protein, Human
氨基酸序列

SAPSSSVPSSSTSPAAFLASGSAQPPPAERCPAACECSEAARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLEALNLSGNHLKEVCAGAFEHLPGLRRLDLSHNPLTNLSAFAFAGSNASVSAPSPLEELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALRSGLALRGLTRLELASNHFLFLPRDLLAQLPSLRYLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLHNSTLAEWHGLAHVKVFLDNNPWVCDCYMADMVAWLKETEVVPDKARLTCAFPEKMRNRGLLDLNSSDLDCDAVLPQSLQTS

分子量

50-70 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TPBG/5T4 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TPBG/5T4 Protein, Mouse (HEK293, His)
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HY-P70121
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