TRAP1 蛋白, Human (GST)

As a chaperone with ATPase activity, TRAP1 protein plays a crucial role in protecting mitochondrial function and polarization, especially downstream of PINK1 and mitochondrial complex I. TRAP1 acts as a negative regulator of mitochondrial respiration, affecting the balance between oxidative phosphorylation and aerobic glycolysis. TRAP1 Protein, Human (GST) is the recombinant human-derived TRAP1 protein, expressed by E. coli , with N-GST labeled tag. The total length of TRAP1 Protein, Human (GST) is 249 a.a., with molecular weight of ~54.5 kDa.

TRAP1（TNF 受体相关蛋白 1）作为具有 ATP 酶活性的伴侣，是维持线粒体功能和极化不可或缺的一部分，在 PINK1 和线粒体复合物 I 的下游发挥作用。它充当线粒体呼吸的负调节因子，动态影响线粒体呼吸之间的平衡。氧化磷酸化和有氧糖酵解。TRAP1 对线粒体呼吸的影响可能是通过调节线粒体 SRC 和抑制 SDHA 介导的。此外，TRAP1 与多种细胞成分相互作用，包括与肿瘤坏死因子 1 型受体的胞内结构域结合、与 RB1 形成复合物以及与 SRC 和 SDHA 结合。这些相互作用强调了 TRAP1 参与细胞过程的多方面性质（参考：未提供具体参考）。

Human

E. coli

N-GST

Q12931 (60S-308E)

10131  [NCBI]

STQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWMMDPKDVRE

Approximately 54.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.