UBASH3A 蛋白, Human (His)

UBASH3A interferes with CBL-mediated downregulation and degradation of receptor-type tyrosine kinases and promotes the accumulation of activating receptors such as T cell receptors, EGFR and PDGFRB on the cell surface. It exhibits minimal protein tyrosine phosphatase activity and may act as a dominant negative regulator of UBASH3B-dependent dephosphorylation. UBASH3A Protein, Human (His) is the recombinant human-derived UBASH3A protein, expressed by E. coli , with N-His labeled tag. The total length of UBASH3A Protein, Human (His) is 270 a.a., with molecular weight of ~30 kDa.

UBASH3A 蛋白破坏 CBL 介导的受体型酪氨酸激酶的下调和降解，导致 T 细胞受体、EGFR 和 PDGFRB 等激活的靶受体在细胞表面积累。尽管在中性 pH 条件下蛋白酪氨酸磷酸酶活性极小，但 UBASH3A 可能充当 UBASH3B 依赖性去磷酸化的显性失活调节因子。此外，UBASH3A 可能通过其 SH3 结构域功能性隔离动力来阻碍动力依赖性内吞途径。它形成同二聚体或同寡聚体并与 CBL 相互作用，在与 CBL 和激活的 EGFR 的复合物中发挥作用。此外，UBASH3A 与泛素和单泛素化蛋白以及动力相互作用，有助于其多方面的调节功能。

Immobilized Human UBASH3A at 2 μg/mL (100 μL/well) can bind Anti-UBASH3A Antibody. The ED₅₀ for this effect is 1.463 μg/mL.

Human

E. coli

N-His

P57075-2 (A354-N623)

53347  [NCBI]

ATVARKSVLVVRHGERVDQIFGKAWLQQCSTPDGKYYRPDLNFPCSLPRRSRGIKDFENDPPLSSCGIFQSRIAGDALLDSGIRISSVFASPALRCVQTAKLILEELKLEKKIKIRVEPGIFEWTKWEAGKTTPTLMSLEELKEANFNIDTDYRPAFPLSALMPAESYQEYMDRCTASMVQIVNTCPQDTGVILIVSHGSTLDSCTRPLLGLPPRECGDFAQLVRKIPSLGMCFCEENKEEGKWELVNPPVKTLTHGANAAFNWRNWISGN

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.