UBE2H 蛋白, Human (GST)

UBE2H Protein, a crucial participant in cellular ubiquitination, transfers ubiquitin from the E1 complex to various proteins, including MAEA of the CTLH E3 ubiquitin-protein ligase complex. Versatile in vitro, UBE2H catalyzes both 'Lys-11'- and 'Lys-48'-linked polyubiquitination, showcasing its involvement in diverse ubiquitin-related processes. Notably, it demonstrates the capability to ubiquitinate histone H2A in vitro. UBE2H Protein, Human (GST) is the recombinant human-derived UBE2H protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2H Protein, Human (GST) is 183 a.a., with molecular weight of ~50.0 kDa.

UBE2H 是细胞泛素化过程的关键参与者，在泛素从 E1 复合物转移到各种蛋白质的过程中发挥着关键作用。特别值得注意的是它能够将泛素转移到 MAEA，MAEA 是 CTLH E3 泛素-蛋白质连接酶复合物的核心成分。体外研究证明了 UBE2H 的多功能性，因为它可以催化“Lys-11”和“Lys-48”连接的多聚泛素化。此外，UBE2H 表现出体外泛素化组蛋白 H2A 的能力，突显其参与多种泛素相关过程。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P62256 (M1-L183)

7328  [NCBI]

MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL

Approximately 50.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.