VEGFR-2 蛋白, Mouse (743a.a, HEK293, Fc)

VEGFR-2 protein is an important tyrosine protein kinase receptor. As a cell surface receptor for VEGFA, VEGFC and VEGFD, it plays an important role in the regulation of angiogenesis, vascular development, permeability and embryonic hematopoiesis. It actively promotes endothelial cell proliferation, survival, migration, differentiation, and actin cytoskeletal reorganization. VEGFR-2 Protein, Mouse (743a.a, HEK293, Fc) is the recombinant mouse-derived VEGFR-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of VEGFR-2 Protein, Mouse (743a.a, HEK293, Fc) is 743 a.a., with molecular weight of ~138.21 kDa.

VEGFR-2 是一种酪氨酸蛋白激酶，是 VEGFA、VEGFC 和 VEGFD 的重要细胞表面受体，在血管生成调节、血管发育、通透性和胚胎造血中发挥重要作用。它积极促进内皮细胞增殖、存活、迁移和分化，以及肌动蛋白细胞骨架的重组。缺乏跨膜结构域的异构体（如异构体 2）可充当诱饵受体，特别是通过限制游离 VEGFA 和/或 VEGFC 的可用性并阻止其与 FLT4 结合来负调节 VEGFA 和 VEGFC 介导的淋巴管生成。VEGFR-2 通过异二聚体形成调节 FLT1 和 FLT4 信号传导。血管生长因子与异构体 1 的结合启动各种信号级联反应，包括 PLCG1 的激活，导致二酰基甘油和肌醇 1,4,5-三磷酸的产生以及蛋白激酶 C 的激活。此外，它还介导MAPK1/ERK2、MAPK3/ERK1、AKT1 和 PIK3R1 的磷酸化，有助于磷脂酰肌醇 3-激酶调节和肌动蛋白细胞骨架重组。VEGFR-2 对于 VEGFA 诱导的 NOS2 和 NOS3 表达不可或缺，从而导致内皮细胞产生一氧化氮，并且它磷酸化 PLCG1、FYN、NCK1、NOS3、PIK3R1、PTK2/FAK1 和 SRC。

Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ this effect is 4.894 ng/mL in the presence of 5 ng/mL recombinant human VEGF165, corresponding to a specific activity is 2.043×10^5 units/mg.

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  Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells.The ED₅₀ for this effect is 4.894 ng/mL in the presence of 5 ng/mL recombinant human VEGF165, corresponding to a specific activity is 2.043×10⁵ units/mg.

Mouse

HEK293

C-hFc

P35918 (A20-E762)

16542  [NCBI]

ASVGLPGDFLHPPKLSTQKDILTILANTTLQITCRGQRDLDWLWPNAQRDSEERVLVTECGGGDSIFCKTLTIPRVVGNDTGAYKCSYRDVDIASTVYVYVRDYRSPFIASVSDQHGIVYITENKNKTVVIPCRGSISNLNVSLCARYPEKRFVPDGNRISWDSEIGFTLPSYMISYAGMVFCEAKINDETYQSIMYIVVVVGYRIYDVILSPPHEIELSAGEKLVLNCTARTELNVGLDFTWHSPPSKSHHKKIVNRDVKPFPGTVAKMFLSTLTIESVTKSDQGEYTCVASSGRMIKRNRTFVRVHTKPFIAFGSGMKSLVEATVGSQVRIPVKYLSYPAPDIKWYRNGRPIESNYTMIVGDELTIMEVTERDAGNYTVILTNPISMEKQSHMVSLVVNVPPQIGEKALISPMDSYQYGTMQTLTCTVYANPPLHHIQWYWQLEEACSYRPGQTSPYACKEWRHVEDFQGGNKIEVTKNQYALIEGKNKTVSTLVIQAANVSALYKCEAINKAGRGERVISFHVIRGPEITVQPAAQPTEQESVSLLCTADRNTFENLTWYKLGSQATSVHMGESLTPVCKNLDALWKLNGTMFSNSTNDILIVAFQNASLQDQGDYVCSAQDKKTKKRHCLVKQLIILERMAPMITGNLENQTTTIGETIEVTCPASGNPTPHITWFKDNETLVEDSGIVLRDGNRNLTIRRVRKEDGGLYTCQACNVLGCARAETLFIIEGAQEKTNLE

Approximately 138.21 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.