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  3. Vps29 蛋白, Human (His, Strep)

Vps29 蛋白, Human (His, Strep)

目录号: HY-P702055
产品使用指南

Vps29 蛋白是逆转录酶货物选择性复合物 (CSC) 的重要组成部分,可防止跨膜货物蛋白错配导致溶酶体降解。它通过涉及 SNX-BAR、SNX3 和 SNX27 逆转录体复合物的不同途径协调从内体到跨高尔基体网络的逆行转运。Vps29 蛋白, Human (His, Strep) 是重组的 Vps29 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。Vps29 蛋白, Human (His, Strep) 全长 182 个氨基酸。

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描述

The Vps29 protein is an important component of the reverse transcriptase cargo-selective complex (CSC), which prevents mispairing of transmembrane cargo proteins leading to lysosomal degradation. It coordinates retrograde transport from endosomes to the trans-Golgi network through distinct pathways involving the SNX-BAR, SNX3, and SNX27 retrosome complexes. Vps29 Protein, Human (His, Strep) is the recombinant human-derived Vps29 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of Vps29 Protein, Human (His, Strep) is 182 a.a., .

研究背景

Vps29 是逆转录酶货物选择性复合物 (CSC) 的关键组成部分,在防止选定的跨膜货物蛋白错配进入溶酶体降解途径中发挥着至关重要的作用。CSC 作为招募中心,通过 SNX-BAR、SNX3 和 SNX27 逆转录体复合物介导的不同逆转录体途径,协调货物蛋白从内体到跨高尔基体网络 (TGN) 的逆行运输。Vps29 是转胞吞作用调节中不可或缺的一部分,其参与聚合免疫球蛋白受体 (pIgR-pIgA) 的修复和再循环就是例证。此外,Vps29 是检索复合体的关键组成部分,其功能独立于逆转录酶,可检索和回收多种货物,包括整合素 alpha-5/beta-1 (ITGA5:ITGB1) 和含有 NxxY 基序的货物蛋白。猎犬复合体向内体膜的募集涉及 CCC 和 WASH 复合体。Vps29 参与 GLUT1 内体到质膜的运输,这取决于它与 ANKRD27 的关联。值得注意的是,在微生物感染的情况下,逆转录体 CSC 介导人乳头瘤病毒从早期内体中退出,并促进递送至高尔基体。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

Q9UBQ0 (M1-P182)

基因 ID

51699

蛋白结构
N-term
Strep-6*His
Vps29 (M1-P182)
Accession # Q9UBQ0
C-term
同用名
VPS29; Vacuolar protein sorting-associated protein 29; hVPS29; PEP11 homolog; Vesicle protein sorting 29
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Vps29 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Vps29 Protein, Human (His, Strep)
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