1. 重组蛋白
  2. Ubiquitin Related Proteins Biotinylated Proteins
  3. Ubiquitin Enzymes
  4. E3 Ligases
  5. X-Linked Inhibitor Of Apoptosis (XIAP)
  6. XIAP 蛋白, Human (Avi)

XIAP 蛋白, Human (Avi)

目录号: HY-P73485
COA 产品使用指南

XIAP 蛋白是一种多功能调节因子,可控制细胞凋亡、炎症、免疫、铜稳态和各种信号通路。作为一种半胱天冬酶抑制剂,它可阻断 CASP3 和 CASP7 底物访问,从而保持 CASP9 不活跃。XIAP 蛋白, Human (Avi) 是重组的 XIAP 蛋白,由 E. coli 表达,带有 C-Avi 标签。XIAP 蛋白, Human (Avi) 全长 236 个氨基酸,分子量约为 ~29.1 kDa。

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规格 价格 是否有货 数量
50 μg ¥4000 In-stock
100 μg   询价  

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描述

XIAP protein is a multifunctional regulator that controls apoptosis, inflammation, immunity, copper homeostasis, and various signaling pathways. As a caspase inhibitor, it blocks CASP3 and CASP7 substrate access, keeping CASP9 inactive. XIAP Protein, Human (Avi) is the recombinant human-derived XIAP protein, expressed by E. coli , with C-Avi labeled tag. The total length of XIAP Protein, Human (Avi) is 236 a.a., with molecular weight of ~29.1 kDa.

研究背景

XIAP 蛋白是一种多功能调节因子,具有多种作用,涵盖细胞凋亡、炎症信号传导、免疫、铜稳态、促有丝分裂激酶信号传导、细胞增殖、侵袭和转移。作为直接 caspase 抑制剂,XIAP 阻止底物进入 CASP3 和 CASP7 的活性位点袋,同时保持 CASP9 处于单体、非活性状态。作为 E3 泛素蛋白连接酶,XIAP 协调多种靶蛋白的泛素化,包括 RIPK1、RIPK2、MAP3K2/MEKK2、DIABLO/SMAC、AIFM1、CCS、PTEN 和 BIRC5/survivin,从而调节 NF-kappa-B 信号传导和其他重要途径。在先天免疫领域,XIAP 介导 NOD1 和 NOD2 下游 RIPK2 的“Lys-63”连接多聚泛素化,激活 NF-kappa-B 和 MAP 激酶信号传导。此外,XIAP 影响 BMP 信号通路、SMAD 和 MAP3K7/TAK1 依赖性通路以及 Wnt 信号级联。它通过泛素化 COMMD1 在铜稳态中发挥作用,并且还在 NEDD8 缀合途径中充当 E3 泛素蛋白连接酶。此外,XIAP 可以保护细胞免受自发性核糖体形成的影响,通过泛素化 RIPK1 和 CASP8 抑制核糖体形成,并通过泛素化 TLE 蛋白正向调节 Wnt 信号传导。这些不同的功能强调了 XIAP 在跨多个信号通路调控细胞过程中的复杂而关键的作用。

生物活性

Measured by its binding ability in a functional ELISA.Immobilized recombinant human SMAC-His at 10 μg/mL (100 μl/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg/mL.

种属

Human

表达系统

E. coli

标签

C-Avi

蛋白编号

P98170 (L121-T356)

基因 ID

331  [NCBI]

蛋白结构
N-term
XIAP (L121-T356)
Accession # P98170
Avi
C-term
同用名
E3 ubiquitin-protein ligase XIAP; ILP; IAP-3; XIAP; API3; BIRC4
分子量

Approximately 29.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 10 mM DTT, 1% Glycerol, 0.2 M Glutamine Potassium, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
XIAP Protein, Human (Avi)
目录号:
HY-P73485
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