ZP3 蛋白, Human (HEK293, His-Myc)

ZP3 protein, a vital component of the zona pellucida, is crucial for sperm binding, inducing the acrosome reaction, and preventing polyspermy. Alongside ZP1, ZP2, and ZP4, it forms a glycoprotein complex in the zona matrix, organizing into filaments cross-linked by ZP1 homodimers. ZP3 actively interacts with ZP1 and ZP2, highlighting its integral role in the molecular architecture governing fertilization processes. ZP3 Protein, Human (HEK293, His-Myc) is the recombinant human-derived ZP3 protein, expressed by HEK293 , with N-His, N-Myc labeled tag.

作为透明带的重要组成部分，ZP3 蛋白有助于卵母细胞周围的细胞外基质，在介导精子结合、诱导顶体反应和防止受精后多受精方面发挥关键作用。在透明带内，ZP3 与 ZP1、ZP2 和 ZP4 一起形成糖蛋白复合物，对于精子结合和透明带基质的形成至关重要。ZP2 和 ZP3 聚合物组织成由 ZP1 同二聚体交联的长丝，突出了透明带基质的结构复杂性。此外，ZP3 与 ZP1 和 ZP2 积极相互作用，强调了其在控制受精过程的复杂分子结构中不可或缺的作用。

Human

HEK293

N-6*His;N-Myc

P21754 (Q23-V387)

7784  [NCBI]

Extracellular Domain

QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSV

Approximately 55-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.