Bathophenanthroline  (Synonyms: 红菲咯啉)

Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru²⁺. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].

在 SDS-PAGE 凝胶染色实验中，含有 Bathophenanthroline (BP) 和 Bathophenanthroline Disulfonate (BPS) 的异配体钌 (II) 络合物在近中性 pH 条件下表现出比 SYPRO Ruby 更高的蛋白质检测灵敏度，减少了人工翻译后修饰^[1]。
PROTOCOL^[1]：
1. 凝胶在 7% 乙酸/10% 乙醇中固定。
2. 使用 0.5-1 mg/L 的 Ru(BPS)₂(BP) 或 Ru(BPS)(BP)₂在酸性/中性缓冲液中过夜染色。
3. 脱色后通过 532 nm 激发/610 nm 发射波长进行荧光扫描。

332.40

C₂₄H₁₆N₂

1662-01-7

固体

Off-white to light yellow

红菲绕啉；浴菲绕啉；红菲咯啉

Room temperature in continental US; may vary elsewhere.

RT, protect from light, stored under nitrogen

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用，-20°C储存时，请在1年内使用。

• [1]. Babak MV, et al. Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining. Inorg Chem. 2020 Apr 6;59(7):4527-4535.  [Content Brief]