AMP-PNP  (Synonyms: Adenylyl imidodiphosphate)

AMP-PNP (Adenylyl imidodiphosphate) is a non-hydrolyzable ATP analog. AMP-PNP binds to ATP binding sites competely but is not hydrolyzed by enzymes, providing stable experimental conditions for studying ATP-dependent processes. AMP-PNP can also be used to study enzyme activity, kinase regulation, DNA/RNA metabolism, ion channel function, and protein complex assembly^[1][2][3][4].

AMP-PNP (100 nM-3.3 mM) 竞争性地从 ATP-琼脂糖珠上洗脱 BtuCD(EQNC)-F，从而抑制野生型和突变型 BtuCD-F^[1] 的 ATPase 活性。
AMP-PNP (1.9-2 mM，0-15 分钟) 无法抑制真核生物 mRNA 5' 前导序列的 ATP 依赖性扫描过程^[2]。
AMP-PNP (1 μM-1 mM) 浓度依赖性地阻断小鼠骨骼肌中的单个 ATP 敏感钾通道 (KATP通道)^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

506.20

C₁₀H₁₇N₆O₁₂P₃

25612-73-1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Korkhov VM, et al. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012 Oct 18;490(7420):367-72.  [Content Brief]

  [2]. Sakharov PA, et al. Nonhydrolyzable ATP analog 5'-adenylyl-imidodiphosphate (AMP-PNP) does not inhibit ATP-dependent scanning of leader sequence of mRNA. Biochemistry (Mosc). 2015 Jan;80(1):45-9.  [Content Brief]

  [3]. Ellinghaus TL, et al. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021 Dec 24;7(52):eabk2392.  [Content Brief]

  [4]. Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP. Eur Biophys J. 1994;23(4):231-7.  [Content Brief]