14-3-3 eta 抗体 (YA838)

14-3-3 eta Antibody (YA838) is a non-conjugated and Mouse origined monoclonal antibody about 28 kDa, targeting to 14-3-3 eta (5F2). It can be used for WB assays with tag free, in the background of Human, Mouse, Rat.

14-3-3 eta (5F2)：该基因产物属于 14-3-3 蛋白家族，通过与含磷酸丝氨酸的蛋白结合来介导信号转导。这种高度保守的蛋白质家族存在于植物和哺乳动物中，并且这种蛋白质与小鼠、大鼠和牛的直系同源物具有 99% 的相同性。该基因在其 5' UTR 中包含一个 7 bp 的重复序列，该重复序列数量的变化与早发性精神分裂症和精神病性双相情感障碍有关。[由 RefSeq 提供，2009 年 6 月]

Free

7533

Predicted band size: 28 kDa;Observed band size: 28 kDa

affinity purified

Non-conjugated

Neuroscience

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat

Q04917

WB: 1:500-1:1,000

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using 14-3-3 eta(HY-P80525) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80525, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human 14-3-3η.The exact sequence is proprietary to MCE.