1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. ABI2 抗体 (YA2840)

ABI2 抗体 (YA2840)

目录号: HY-P83095
COA 抗体使用指南 技术支持

ABI2 Antibody (YA2840) 是一个兔来源、无偶联标记、抗 ABI2 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ABI2 Antibody (YA2840) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABI2.

研究背景

ABI2 是调控细胞运动和粘附过程中肌动蛋白细胞骨架(actin cytoskeleton)动态的关键因子。作为 WAVE 复合体(WAVE complex)的组成成分,它通过激活肌动蛋白成核机器 Arp2/3 促进片状伪足(lamellipodia)形成。同时,ABI2 作为非受体酪氨酸激酶 ABL1 和 ABL2 的调节因子和底物,参与细胞生长和分化相关过程(by similar)。在神经元中,它调控树突棘(dendritic spine)形态发生,可能促进与记忆相关的大型蘑菇型树突棘(mushroom-type spines)形成(by similar)。此外,ABI2 参与上皮细胞新生粘附连接(adherens junction)组装,并在晶状体形态发生中可能通过调控片状伪足驱动的粘附连接形成发挥作用(by similar)。

基因 ID
蛋白数据库
中文名
ABI2 抗体 (YA2840)
同用名
Abelson interactor 2; ABI2; ABI2B; Abl binding protein 3; AblBP3; ArgBP1; ARGBPIA; ArgBPIB; SSH3BP2
分子量

Predicted band size: 56 kDa; Observed band size: 75 kDa

纯度

Affinity Purified

偶联

Non-conjugated

修饰

Unmodified

研究领域

Cancer

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

  • Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), Hela (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ABI2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
应用

WB, IHC-P, ICC/IF

性状

液体

组分

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthesized peptide derived from human ABI2

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
ABI2 Antibody (YA2840)
目录号:
HY-P83095
需求量: