1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. Aconitase 2 抗体 (YA2151)

Aconitase 2 抗体 (YA2151)

目录号: HY-P82406
COA 抗体使用指南 技术支持

Aconitase 2 Antibody (YA2151) 是一个兔来源、无偶联标记、抗 Aconitase 2 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Aconitase 2 Antibody (YA2151) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aconitase 2.

研究背景

Aconitase 2 是一种含 [4Fe-4S] 簇的铁硫酶(iron-sulfur enzyme),通过顺乌头酸(cis-aconitate)中间体催化柠檬酸(citrate)与异柠檬酸(isocitrate)的相互转化,在三羧酸循环(TCA cycle)和乙醛酸循环(glyoxylate cycle)中起关键作用。该酶对氧化损伤高度敏感,其 [4Fe-4S] 簇易被超氧阴离子(superoxide)、H2O2 和 NO 等活性氧/氮物种破坏,导致铁离子释放并加剧细胞氧化应激。Aconitase 2 的活性受镁离子浓度和柠檬酸/异柠檬酸比例调控,硫氧还蛋白(thioredoxin)可修复其氧化损伤后的活性中心。

基因 ID
蛋白数据库
中文名
Aconitase 2 抗体 (YA2151)
同用名
Aconitate hydratase; mitochondrial; Aconitase; Citrate hydro-lyase
分子量

Predicted band size: 85 kDa; Observed band size: 85 kDa

纯度

Affinity Chromatography

偶联

Non-conjugated

修饰

Unmodified

研究领域

Tags & Cell Markers

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:50

  • Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Mouse liver (lane 5, 15 μg) , Rat liver (lane 5, 15 μg) using Aconitase 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
应用

WB, IHC-P, ICC/IF, IP

性状

液体

组分

Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthesized peptide derived from human Aconitase 2

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Aconitase 2 Antibody (YA2151)
目录号:
HY-P82406
需求量: