ADH5 抗体 (YA1998)

ADH5 Antibody (YA1998) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ADH5.

ADH5 是一种 III 类乙醇脱氢酶，通过催化长链伯醇和 S-（羟甲基）谷胱甘肽的氧化，在细胞解毒过程中发挥关键作用。此外，ADH5 还参与长链 omega-羟基脂肪酸（例如 20-羟基二十碳四烯酸 (20-HETE)）的氧化，产生中间体醛、20-氧代花生四烯酸和最终产物二羧酸（ 5Z,8Z,11Z,14Z)-二十碳四烯二酸酯。尽管 ADH5 被归类为乙醇脱氢酶，但其氧化乙醇的效率较低。值得注意的是，ADH5 对于细胞防御甲醛（一种细胞毒性和致癌代谢物）至关重要，有助于清除这种物质并防止潜在的 DNA 损伤。

P11766

Predicted band size: 40 kDa; Observed band size: 40 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104365

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IP: 1/20

• 
  Western blot analysis of extracts from MCF-7 (lane 2(20μg), K562 (lane 3(20μg) and HepG2 (lane 4(20μg) using ADH5 (HY-P82253) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human ADH5