AKT1+AKT2+AKT3 抗体 (YA635)

AKT Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 56 kDa, targeting to AKT. It can be used for WB,IHC-F,IHC-P,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat, Hamster.

AKT1 蛋白与 AKT2 和 AKT3 一样是 AKT 激酶家族的成员，在调节代谢、增殖、细胞存活、生长和血管生成等多种细胞过程中发挥着关键作用。AKT1 通过众多下游底物的丝氨酸和/或苏氨酸磷酸化发挥作用，参与 100 多种报道的候选底物的调节，这证明了其多方面的性质。值得注意的是，它协调胰岛素诱导的 SLC2A4/GLUT4 葡萄糖转运蛋白易位，影响葡萄糖摄取。此外，AKT1 通过磷酸化 GSK3A 和 GSK3B 来控制葡萄糖以糖原形式储存，抑制其激酶活性。它参与多种途径，包括通过 mTORC1 信号途径介导胰岛素刺激的蛋白质合成以及调节 FOXO 因子，影响细胞定位。AKT1 还影响 NF-kappa-B 依赖性基因转录并正向调节 CREB1 活性，有助于促生存基因的转录。此外，AKT1 通过磷酸化 ATP 柠檬酸裂解酶 (ACLY) 并激活环核苷酸磷酸二酯酶 (PDE3B) 的 3B 同工型，从而在脂质代谢中发挥作用，从而降低环 AMP 水平并抑制脂肪分解。除了这些功能之外，AKT1 还参与细胞迁移、粘附组装和分解、胎盘发育以及对生长因子的多种细胞反应，包括血小板源性生长因子 (PDGF)、表皮生长因子 (EGF)、胰岛素和胰岛素样因子生长因子 I (IGF-I)。最近发现 AKT1 在 SPATA13 介导的细胞迁移和粘附调节中的作用增加了其功能的复杂性，突出了 AKT1 在细胞稳态和对环境信号的响应中的核心作用。

Free

P31749 / P31751 / Q9Y243

Predicted band size: 56 kDa; Observed band size: 56 kDa

affinity purified

Non-conjugated

Unmodified

AB_3694201

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat, Hamster

WB: 1:500-1:1,000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Western blot analysis of extracts from Jurkat (lane 2(20μg), Hela (lane 3(20μg) and C6(lane 4(20μg) using AKT1/AKT2/AKT3 (HY-P80535) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using AKT1/AKT2/AKT3 Antibody (YA635). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human AKT1/2/3 aa:281-480.