ATF4 抗体 (YA605)

ATF4 Antibody (YA605) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATF4.

ATF4 是一种转录因子，能够结合 cAMP 响应元件 (CRE) (共识序列: 5'-GTGACGT[AC][AG]-3')，并在正常细胞条件下调控代谢和氧化还原过程，同时在整合应激响应 (ISR) 中作为主转录因子发挥作用。它能够以异源二聚体形式结合不对称 CRE，或以同源二聚体形式结合回文 CRE (By similarity)。作为 ISR 的核心效应因子，ATF4 在应对内质网 (ER) 应激、氨基酸饥饿、线粒体应激或氧化应激等条件下发挥关键作用。在 ISR 期间，ATF4 的翻译通过 EIF2S1/eIF-2-alpha 磷酸化诱导的替代核糖体翻译重起始机制上调，并通过激活应激响应基因的转录促进细胞恢复。此外，ATF4 还参与调控氨基酸充足性和抗氧化应激相关基因的转录 (By similarity)。在 ER 应激条件下，ATF4 可能与其他因子协同激活 NLRP1 的转录，并响应氨基酸剥夺或 ER 应激激活天冬酰胺合成酶 (ASNS) 的表达。然而，当与 DDIT3/CHOP 结合时，ATF4 对 ASNS 基因的转录激活在氨基酸剥夺条件下会受到抑制。ATF4 还与 DDIT3/CHOP 共同调控细胞凋亡相关基因的表达，参与终末未折叠蛋白响应 (terminal UPR) 过程 (By similarity)。此外，ATF4 通过激活 COX7A2L/SCAF1 的表达促进呼吸链超复合物的形成，并在线粒体氧化磷酸化中发挥作用。在无应激条件下，ATF4 的翻译水平较低，但仍参与胚胎晶状体形成、胎儿肝脏造血、骨骼发育和突触可塑性等正常代谢过程 (By similarity)。在成骨细胞中，ATF4 通过 RPS6KA3/RSK2 的磷酸化增强其转录激活活性，并调控骨特异性基因的表达。此外，ATF4 与 FOXO1 协同调控葡萄糖稳态，并通过抑制 β 细胞生成和胰岛素产生发挥作用 (By similarity)。ATF4 还参与调控 SIRT4 的转录，并在昼夜节律调控中发挥作用，周期性激活 SLC6A4 和 PER2 基因的转录 (By similarity)。尽管 ATF4 主要在细胞应激适应中作为转录激活因子发挥作用，但它也可以作为转录抑制因子调控突触可塑性和长期记忆的形成。在神经元中，ATF4 通过与 DISC1 的相互作用抑制其转录因子活性 (Microbial infection) ATF4 还能够结合 HTLV-I 长末端重复序列中的 Tax 响应增强子元件。

Free

P18848

Predicted band size: 39 kDa；Observed band size: 55 kDa

Protein A affinity purified.

Nucleus, Nucleus speckle, Cell membrane, Cytoplasm, Centrosome.

Non-conjugated

Unmodified

AB_3102559

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Immunocytochemistry analysis of Neuro-2a cells labeling ATF4 with ATF4 Antibody (HY-P80486) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATF4 Antibody (HY-P80486) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Neuro-2a cells labeling ATF4 with ATF4 Antibody (HY-P80486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATF4 Antibody (HY-P80486)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using ATF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80486, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using ATF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80486, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human ATF4.AA range:1-220.