c-Fos 抗体 (YA506)

c-Fos Antibody (YA506) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to c-Fos.

c-Fos 是一种核磷蛋白，与 JUN/AP-1 转录因子形成紧密但非共价连接的复合物。在异源二聚体中，FOS 和 JUN/AP-1 的基本区域各自与对称的 DNA 半位点相互作用。在 TGF-beta 激活时，c-Fos 在 AP1/SMAD 结合位点形成多聚体 SMAD3/SMAD4/JUN/FOS 复合物，以调节 TGF-beta 介导的信号传导。它在调控形成和维持骨骼的细胞发育中具有关键作用，被认为在信号转导、细胞增殖和分化中发挥重要作用。在生长细胞中，c-Fos 可能通过激活 CDS1 和 PI4K2A 来激活磷脂合成，这一活性需要 Tyr 去磷酸化并与内质网结合。

Free

P01100

Predicted band size: 41 kDa; Observed band size: 41/55 kDa

Protein A affinity purified.

Nucleus, Endoplasmic reticulum, Cytoplasm.

Non-conjugated

Unmodified

AB_3102070

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500 ;IHC-P: 1:50-1:200 ;ICC/IF: 1:100

• 
  Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Immunohistochemical analysis of paraffin-embedded human ‌Cervical Carcinoma tissue using c-Fos antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using c-Fos antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80081, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

WB, IHC-P, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human c-Fos.AA range:231-268.