Caspase 11 抗体 (YA562)

Caspase 11 Antibody (YA562) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase 11.

Caspase 11：该基因编码在细胞凋亡、细胞迁移和炎症反应中起重要作用的半胱氨酸蛋白酶成员。编码的蛋白质介导巨噬细胞在细菌感染后产生促炎细胞因子。缺乏编码蛋白的小鼠对脂多糖诱导的内毒素休克有抵抗力。已经描述了包含剪接受体连接的 5-bp 缺失，导致某些小鼠品系中的交替剪接和较短的非功能性亚型。尽管其官方命名为“caspase 4，细胞凋亡相关的半胱氨酸肽酶”，但该基因及其编码的蛋白质在历史上被称为 caspase 11。该基因存在于 9 号染色体上的三个 caspase 基因簇中。[由 RefSeq 提供，4 月2015]

Free

P70343

Predicted band size: 43 kDa;Observed band size: 43,38,25 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102139

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IP: 1:20

• 
  Western blot analysis of extracts from C6(lane 2(20μg) ,NIH/3T3(lane 3(20μg) and Hela(lane 4(20ug)using Caspase 11 Antibody (HY-P80588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Mouse Caspase-11.The exact sequence is proprietary to MCE.