COUP TF1 抗体 (YA2322)

COUP TF1 Antibody (YA2322) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COUP TF1.

COUP TF1 (chicken ovalbumin upstream promoter) 是一种转录因子，能够与 ovalbumin 启动子结合，并与另一种蛋白 S300-II 协同作用，刺激转录的起始。它能够结合到 5'-AGGTCA-3' 基序的直接重复序列和回文序列上。此外，COUP TF1 还能够抑制 LHCG 的转录活性。

P10589

Predicted band size: 46 kDa; Observed band size: 46 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104678

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;FC: 1/50-1/100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using COPS3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using COUP TF1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using COUP TF1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human COUP TF1