CRM1 抗体 (YA1554)

目录号: HY-P81809: Data Sheet SDS COA 抗体使用指南
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CRM1 Antibody (YA1554) 是一个兔来源、无偶联标记、抗 CRM1 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥470	In-stock
50 μL	￥1250	In-stock
100 μL	￥2000	In-stock
250 μL		询价

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CRM1 Antibody (YA1554) 相关产品:

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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描述

CRM1 Antibody (YA1554) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRM1.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量

Predicted band size: 123 kDa;

Observed band size: 110 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

O14980

基因 ID

7514 [NCBI]

免疫原

A synthesized peptide derived from human CRM1 aa1000-1071.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
IHC	1:50-1:100
IF	1:50-1:200
FC FC: 流式细胞术	1:50-1:100

敏感性	Endogenous	纯度	Affinity Chromatography
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC FC

Western blot analysis of extracts from Hela(lane 2(20ug) , PANC-1(lane 3(20ug) and NIH/3T3(lane 4(20ug) using CRM1 Antibody (HY-P81809) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling CRM1 with CRM1 Antibody (HY-P81809)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CRM1 Antibody (HY-P81809) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A431 cells labeling CRM1 with CRM1 Antibody (HY-P81809) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CRM1 Antibody (HY-P81809) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Flow cytometric analysis of 1X10^6 Hela cells labeling CRM1 Antibody (YA1554) (HY-P81809, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

RRID

AB_3103141

反应种属数据库

Entrez Gene: 7514 Human ; 103573 Mouse ; 85252 Rat

SwissProt: O14980 Human ; Q6P5F9 Mouse ; Q80U96 Rat

OMIM: 602559 Human

研究领域

Signal Transduction

中文名

CRM1 抗体 (YA1554)

同用名

XPO1; CRM1; Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

CRM1 Antibody (YA1554) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

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CRM1 抗体 (YA1554)

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CRM1 抗体 (YA1554)

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