1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. Cytochrome P450 2E1 抗体 (YA2302)

Cytochrome P450 2E1 抗体 (YA2302)

目录号: HY-P82557
COA 抗体使用指南 技术支持

Cytochrome P450 2E1 Antibody (YA2302) 是一个兔来源、无偶联标记、抗 Cytochrome P450 2E1 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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描述

Cytochrome P450 2E1 Antibody (YA2302) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 2E1.

研究背景

Cytochrome P450 2E1 是一种参与脂肪酸代谢的 cytochrome P450 monooxygenase (PubMed:10553002,PubMed:18577768)。其作用机制是利用分子氧,将一个氧原子插入底物,并将第二个氧原子还原为水分子,同时通过 cytochrome P450 reductase (NADPH--hemoprotein reductase) 从 NADPH 获取两个电子 (PubMed:10553002,PubMed:18577768)。它能催化碳氢键的羟基化反应,尤其擅长在饱和脂肪酸的 omega-1 位点进行羟基化 (PubMed:10553002,PubMed:18577768)。此外,它可能参与外源性物质的氧化代谢 (Probable)。by similar: 其他 cytochrome P450 家族成员也可能具有类似的底物广谱性。

基因 ID
蛋白数据库
中文名
Cytochrome P450 2E1 抗体 (YA2302)
同用名
CYP2E1; CYP2E; Cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-J
分子量

Predicted band size: 57 kDa; Observed band size: 57 kDa

纯度

Affinity Chromatography

偶联

Non-conjugated

修饰

Unmodified

研究领域

Cardiovascular

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:500-1:1000

  • Western blot analysis was performed on extracts from Rat liver (lane 1, 15 μg), and MCF-7 (lane 2, 15 μg) using Cytochrome P450 2E1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
应用

WB

性状

液体

组分

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthesized peptide derived from human CYP2E1

数据库
文件资料

Cytochrome P450 2E1 Antibody (YA2302) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Cytochrome P450 2E1 Antibody (YA2302)
目录号:
HY-P82557
需求量: