Cytokeratin 2e 抗体 (YA2671)

Cytokeratin 2e Antibody (YA2671) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 2e.

Cytokeratin 2e 是一种角蛋白，可能有助于终末角质化。它与 keratinocyte 的激活、增殖和角化相关，对于维持耳部表皮中 suprabasal keratinocytes 的 corneocytes 和 keratin filaments 是必需的，可能通过调节角蛋白及其伴侣蛋白的表达和定位来实现。此外，它在 plantar skin 上表皮屏障的建立中也发挥作用（By similarity）。

P35908

Predicted band size: 65 kDa; Observed band size: 65 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from HaCaT (lane 1, 15 μg) using Cytokeratin 2e Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P

液体

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Cytokeratin 2e