DDX58 抗体 (YA2318)

DDX58 Antibody (YA2318) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDX58.

DDX58 蛋白作为先天免疫受体，对于检测细胞质病毒核酸至关重要，启动下游信号级联，导致 I 型干扰素和促炎细胞因子的产生。它与病毒 RNA 形成核糖核蛋白复合物，促进同源寡聚化成丝状。这种寡聚化促进了 RNF135（一种 E3 泛素蛋白连接酶）的募集，从而放大了 RIG-I 介导的抗病毒信号传导。激活后，DDX58 与 MAVS/IPS1 结合，激活 TBK1 和 IKBKE 激酶，导致 IRF3 和 IRF7 磷酸化。这种激活促进抗病毒免疫基因的转录，包括干扰素 IFN-α 和 IFN-β。DDX58 可识别各种病毒 RNA 配体，例如 5'-三磷酸化 ssRNA 和 dsRNA，其中 5'-三磷酸部分和平端碱基配对对其活性至关重要。该蛋白可检测多种 RNA 病毒，并在针对含 dsDNA 病毒（例如 Epstein-Barr 病毒）的抗病毒信号传导中发挥作用。此外，它还参与粒细胞产生、分化、细菌吞噬作用和细胞迁移的调节。

O95786

Predicted band size: 107 kDa; Observed band size: 107 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104674

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IP: 1:50

• 
  Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using DDX58 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human DDX58