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  2. 一抗
  3. 单克隆抗体 重组抗体
  4. DKC1 抗体 (YA1750)

DKC1 抗体 (YA1750)

目录号: HY-P82005
COA 抗体使用指南 技术支持

DKC1 Antibody (YA1750) 是一个兔来源、无偶联标记、抗 DKC1 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

DKC1 Antibody (YA1750) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DKC1.

研究背景

DKC1 是 H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) 复合物的催化亚基,负责催化 rRNA 的假尿苷化(pseudouridylation),这一过程涉及尿苷的异构化,使得核糖随后连接到 C5 而非正常的 N1。每个 rRNA 可包含多达 100 个假尿苷('psi')残基,可能有助于稳定 rRNA 的构象。DKC1 是核糖体生物合成和端粒维持所必需的,同时也参与端粒酶逆转录酶(TERT)全酶 RNA 组分 TERC 的正确加工或核内运输。此外,DKC1 促进细胞间及细胞与基质的黏附,提高细胞增殖速率并导致细胞角蛋白(cytokeratin)的过度表达。

基因 ID
蛋白数据库
中文名
DKC1 抗体 (YA1750)
同用名
DKC1; NOLA4; H/ACA ribonucleoprotein complex subunit 4; CBF5 homolog; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1
分子量

Predicted band size: 58 kDa; Observed band size: 58 kDa

纯度

Affinity Chromatography

偶联

Non-conjugated

修饰

Unmodified

研究领域

Epigenetics and Nuclear Signaling

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

  • Western blot analysis was performed on extracts from SW480 (lane 1, 20 μg), HeLa (lane 2, 20 μg), HepG2 (lane 3, 20 μg), and Jurkat (lane 4, 20 μg) using DKC1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
应用

WB, IHC-P, ICC/IF

性状

液体

组分

Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

A synthesized peptide derived from human DKC1

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
DKC1 Antibody (YA1750)
目录号:
HY-P82005
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