EHMT2 抗体 (YA2373)

EHMT2 Antibody (YA2373) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2373), targeting EHMT2, with a predicted molecular weight of 132 kDa (observed band size: 170 kDa). EHMT2 Antibody (YA2373) can be used for WB, IHC-P experiment in human background.

EHMT2 是一种组蛋白甲基转移酶，专门在常染色质中对组蛋白 H3 的 'Lys-9' 进行单甲基化 (H3K9me1) 和二甲基化 (H3K9me2)。H3K9me 通过招募 HP1 蛋白到甲基化组蛋白上，作为表观遗传转录抑制的特异性标记。此外，EHMT2 在 G1 期介导组蛋白 H3 的 'Lys-56' 单甲基化 (H3K56me1)，促进组蛋白 H3 与 PCNA 的相互作用并调节 DNA 复制。它还能微弱地甲基化组蛋白 H3 的 'Lys-27' (H3K27me)。EHMT2 是 DNA 甲基化所必需的，但其组蛋白甲基转移酶活性对 DNA 甲基化并非必需，表明这两种功能是独立运作的。它可能通过 E2F6、MGA、MAX 和/或 DP1 等 DNA 结合蛋白靶向组蛋白 H3，并可甲基化组蛋白 H1。除组蛋白甲基转移酶活性外，EHMT2 还能甲基化非组蛋白，如介导 p53/TP53 的 'Lys-373' 二甲基化，并甲基化 CDYL、WIZ、ACIN1、DNMT1、HDAC1、ERCC6、KLF12 及自身。

Q96KQ7

Predicted band size: 132 kDa; Observed band size: 170 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104728

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100

• 
  Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), 293(lane 2, 20 μg), and A431 (lane 3, 20 μg) using EHMT2/G9A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05%BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human EHMT2/G9A