Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) 抗体 (YA455)

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) is a non-conjugated and Rabbit origined monoclonal antibody about 41/43 kDa, targeting to Erk1 (pT202/pY204) + Erk2 (pT185/pY187). It can be used for WB,IP,IHC-P assays with tag free, in the background of Human.

ERK1 是一种丝氨酸/苏氨酸激酶，是 MAP 激酶信号转导途径的重要组成部分，与 MAPK1/ERK2 协同协调 MAPK/ERK 级联。该通路由 KIT 和 KITLG/SCF 等刺激激活，在细胞生长、粘附、存活和分化等多种生物功能中发挥着关键作用。ERK1 与 MAPK1/ERK2 一起影响转录、翻译和细胞骨架重排，同时还通过众多转录因子的磷酸化促进分化细胞中减数分裂、有丝分裂和有丝分裂后功能的启动和调节。已鉴定出大约 160 种 ERK 底物，其靶点跨越不同的细胞区室，参与转录调控、翻译、有丝分裂和细胞凋亡等过程。此外，MAPK/ERK 级联还涉及内体动力学、溶酶体加工、通过核周回收室 (PNRC) 的内体循环以及有丝分裂期间高尔基体的碎片。值得注意的底物包括转录因子、细胞骨架元件、细胞凋亡调节因子、翻译调节因子以及多种信号相关分子、蛋白激酶和磷酸酶，从而扩展了级联的特异性并放大了其对细胞反应的影响。

Free

P27361 / P28482

Predicted band size: 41/43 kDa; Observed band size: 41/43 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

AB_3102477

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:5,000-1:10,000； IF-Cell: 1:100; IHC-P: 1:1,000; IF-Tissue: 1:200; FC: 1:1,000; IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
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  Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IHC-P, ICC/IF, IP, FC, IF-Tissue

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human.AA range:166-215.