1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. I-FABP 抗体 (YA3770)

I-FABP 抗体 (YA3770)

目录号: HY-P84073
COA 抗体使用指南 技术支持

I-FABP Antibody (YA3770) 是一个小鼠来源、无偶联标记、抗 I-FABP 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥580 In-stock
50 μL ¥1500 In-stock
100 μL ¥2400 In-stock
250 μL   询价  

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Compound Screening Library

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

I-FABP Antibody (YA3770) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to I-FABP.

研究背景

FABP2 是一种脂肪酸结合蛋白,被认为在长链脂肪酸及其酰基辅酶 A 酯的细胞内转运中发挥作用。它可能参与富含甘油三酯的脂蛋白合成,并以高亲和力结合饱和长链脂肪酸,但对不饱和长链脂肪酸的亲和力较低。此外,FABP2 可能通过作为脂质传感器来帮助维持能量稳态。

基因 ID
蛋白数据库
中文名
I-FABP 抗体 (YA3770)
同用名
FABPI; I-FABP; MGC133132; FABP2
分子量

Predicted band size: 15 kDa; Observed band size: 15 kDa

纯度

affinity purified.

偶联

Non-conjugated

产品类别

Primary Antibody; Mouse Monoclonal Antibody

克隆性

Monoclonal Antibody

宿主

Mouse

反应物种

Human

推荐稀释比例

WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

  • Immunocytochemistry analysis of HepG2 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Immunocytochemistry analysis of A431 cells labeling FABP2 with FABP2 Antibody (HY-P84073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP2 Antibody (HY-P84073)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
应用

WB, IHC-P, ICC/IF, FC, ELISA

性状

液体

组分

Supplied in ascitic fluid containing 0.03% sodium azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG1

免疫原

Purified recombinant fragment of human FABP2 full(aa 1-132).

文件资料

I-FABP Antibody (YA3770) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
I-FABP Antibody (YA3770)
目录号:
HY-P84073
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