FKBP51 抗体

FKBP51 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 51 kDa, targeting to FKBP51. It can be used for WB,IHC-P,IP,FC assays with tag free, in the background of Human, Rat.

Free

Predicted band size: 51 kDa;Observed band size: 55 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Rabbit

Human, Rat

SwissProt: Q13451 Human;SwissProt: Q5U2T9 Rat

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Jurkat(lane 2(15μg) or lane 3(30μg)) and Hela(lane 4(15μg) or lane 5(30μg)) using FKBP51(HY-P80401) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80401, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human FKBP51.AA range:400-440.