FOXO1A 抗体 (YA429)

FOXO1A Antibody (YA429) is a non-conjugated and Rabbit origined monoclonal antibody about 70 kDa, targeting to FOXO1A. It can be used for WB,ICC,IHC-P assays with tag free, in the background of Human, Mouse.

FoxO1 是一种转录因子，作为胰岛素信号传导的主要靶点，在氧化应激反应中调节代谢稳态。它能够与胰岛素反应元件（IRE）和 Daf-16 家族结合元件（DBE）结合，其活性受胰岛素抑制。FoxO1 是氧化还原平衡和成骨细胞数量的主要调节因子，控制骨量（By similarity）。它还协调骨骼的内分泌功能以调节葡萄糖代谢（By similarity）。在脂质可用性条件下，FoxO1 作为骨骼祖细胞软骨形成的关键调节因子：当脂质水平低时，它转位至细胞核并促进 SOX9 的表达，从而诱导软骨形成并抑制脂肪酸氧化（By similarity）。FoxO1 与 ATF4 协同抑制骨钙素（BGLAP）的活性，增加血糖水平并引发葡萄糖不耐受和胰岛素抵抗（By similar）。此外，它还能抑制 RUNX2 的转录活性（By similarity）。在胰腺β细胞中，FoxO1 通过抑制 PDX1 的表达来抑制葡萄糖感知（By similarity）。在肝细胞中，FoxO1 与 PPARGC1A 和 CEBPA 共同激活 IGFBP1、G6PC1 和 PCK1 等基因的表达以促进糖异生（By similarity）。FoxO1 还通过直接促进 PPARGC1A 和 G6PC1 的表达来促进糖异生。它是细胞死亡的重要调节因子，作用于 CDK1、PKB/AKT1 和 STK4/MST1 下游（By similarity）。FoxO1 促进神经细胞死亡，并在脂肪组织中调节胰岛素作用（By similarity）。它通过调节 PPARG 等脂肪生成基因的表达来影响前脂肪细胞分化，并在过量热量摄入时调节脂肪细胞大小和脂肪组织特异性基因表达（By similarity）。FoxO1 还调节 GADD45A 的转录活性及β细胞中一氧化氮损伤 DNA 的修复（By similarity）。在心肌细胞肥大和心脏重塑中，FoxO1 介导 MLIP 的功能（By similarity）。在心脏平滑肌细胞中，FoxO1 通过转录激活促凋亡基因来促进凋亡（By similarity）。在内皮细胞（EC）中，FoxO1 通过转录 CCL2 和 BCL2L11 来调节细胞存活和凋亡，这些基因参与 EC 趋化和凋亡（By similar）。

Free

Q12778

Predicted band size: 70 kDa;Observed band size: 100 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102088

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000-1:2,000 ;ICC: 1:50-1:200 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of A549 cells labeling FOXO1A with FOXO1A antibody (HY-P80132) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXO1A antibody (HY-P80132) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HeLa cells labeling FOXO1A with FOXO1A antibody (HY-P80132) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXO1A antibody (HY-P80132) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FOXO1A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80132, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using FOXO1A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80132, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human FOXO1A.AA range:301-350.