G3BP 抗体 (YA2432)

G3BP Antibody (YA2432) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to G3BP.

G3BP 是一种多功能蛋白，参与 stress granule 形成、先天免疫等过程。它在 stress granule 形成中起关键作用，stress granule 是无膜细胞器，在应激反应中储存 mRNA 和蛋白质。G3BP 通过 liquid-liquid phase separation (LLPS) 机制促进 stress granule 形成，作为分子开关响应细胞内游离 RNA 浓度升高触发 RNA 依赖性 LLPS。同时，G3BP 具有 ATP 和镁依赖性解旋酶活性，可高效解旋 DNA/DNA、RNA/DNA 和 RNA/RNA 底物，并沿单链 DNA 单向移动。在先天免疫中，G3BP 通过增强 CGAS 和 RIGI 活性发挥作用，促进 DNA 触发的 cGAS/STING 通路和 RIGI 诱导的 I 型干扰素产生。此外，G3BP 在体外可能作为磷酸化依赖性序列特异性内切核糖核酸酶，特异性切割 MYC mRNA 的 3'-UTR 区域。by similar: G3BP 还参与 cGAS 的凝聚过程，这可能与无膜细胞器的形成有关。

Q13283

Predicted band size: 52 kDa; Observed band size: 68 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104787

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug)using G3BP Antibody (HY-P82687) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of C6 cells labeling G3BP with G3BP Antibody (HY-P82687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with G3BP Antibody ((HY-P82687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Neuro-2a cells labeling G3BP with G3BP Antibody (HY-P82687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with G3BP Antibody ((HY-P82687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using G3BP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using G3BP Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, IP, FC

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human G3BP aa401-466/466.