GAPDH 抗体 (HRP) (YA874)

GAPDH Antibody (HRP) (YA874) is a HRP-conjugated, mouse-derived, anti-GAPDH (HRP) (YA874) IgG1 monoclonal antibody.

GAPDH 蛋白具有双重功能，具有 3-磷酸甘油醛脱氢酶和亚硝基酶活性，分别在糖酵解和核功能中发挥着不可或缺的作用。在糖酵解中，它作为关键酶催化初始步骤，将 D-甘油醛 3-磷酸 (G3P) 转化为 3-磷酸-D-甘油酰磷酸。除了其代谢作用之外，GAPDH 还调节细胞骨架的组织和组装，并通过刺激 CHP1 与微管的结合（通过相似性）促进 CHP1 依赖性微管和膜的关联。它也是步态复合体的一个组成部分，在炎症过程中介导干扰素γ诱导的转录选择性翻译抑制。在先天免疫中，GAPDH 分别通过与 TRAF2 和 TRAF3 相互作用（通过相似性）促进 TNF 诱导的 NF-κ-B 激活和 I 型干扰素产生。此外，它参与核事件，包括转录、RNA 转运、DNA 复制和细胞凋亡，可能是由其亚硝基酶活性介导的，导致核靶蛋白如 SIRT1、HDAC2 和 PRKDC 发生半胱氨酸 S-亚硝基化（通过相似性）。GAPDH 的多方面功能强调了其在细胞过程中的多功能和关键作用。

P04406

Predicted band size: 36 kDa; Observed band size: 36 kDa

Affinity Purified

HRP

Unmodified

AB_3103031

Neuroscience

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:1000-1:5000; ELISA: 1:10000

• 
  Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
• 
  Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 3T3 (lane 3, 15 μg), C6 (lane 4, 15 μg), and RAW264.7 (lane 5, 15 μg), using GAPDH Mouse mAb (HRP).Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:10000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ELISA

液体

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

A synthetic peptide corresponding to residues near the amino terminus of human GAPDH protein.